Stephen J. Valentine, Ph.D.
About
Ph.D., Indiana University
Dr. Valentine's research interests include the development of novel gas-phase separations techniques that can be combined with mass spectrometry (MS). This work is centered on the technique known as ion mobility spectrometry (IMS).
Congratulations to Steve Valentine (PI) and Glen Jackson (co-PI) for receiving a grant in the amount of $1,376,751 from the National Institutes of Health to support a research project entitled "Developing IMS-SID/MAD-MS Instrumentation for Characterizing Intrinsically Disordered Protein Structure".
Teaching Fields
Analytical Chemistry
CHEM 313 Instrumental Analysis Lab
CHEM 312 Environmental Chemistry
CHEM 750 Introduction to Proteomics
CHEM 514 Mass Spectrometry: Principles and Practice
Publications
Khakinejad, M.; Ghassabi Kondalajii, S.; Donohoe, G. C.; Valentine, S. J. Ion Mobility Spectrometry-Hydrogen Deuterium Exchange Mass Spectrometry of Anions: Part 3. Estimating Surface Area Exposure by Deuterium Uptake. J. Am. Soc. Mass Spectrom. 2016, 27(3), 462-473.
Khakinjad, M.; Ghassabi Kondalajii, S.; Donohoe, G. C.; Valentine, S. J. Ion Mobility Spectrometry-Hydrogen Deuterium Exchange Mass Spectrometry of Anions: Part 2. Assessing Charge Site Location and Isotope Scrambling. J. Am. Soc. Mass Spectrom. 2016, 27(3), 451-461.
Chaibva, M.; Jawahery, S.; Pilkington IV, A. W.; Arndt, J. R.; Sarver, O.; Valentine, S. J. Matysiak, S.; Legleiter, J. “Acetylation within the first 17 residues of Huntingtin exon 1 alters aggregation and lipid binding” Biophys. J. 2016, 111(2), 349-362.
Maurer, M. M.; Donohoe, G. C.; Maleki, H.; Yi J.; McBride, C.; Nurkiewicz, T. R.; Valentine, S. J. Comparative plasma proteomic studies of pulmonary TiO2 nanoparticle exposure in rats using liquid chromatography tandem mass spectrometry. J Proteomics 2016, 130, 85-93.
Maurer, M. M.; Donohoe, G. C.; Valentine, S. J. Advances in ion mobility-mass spectrometry instrumentation and techniques for characterizing structural heterogeneity. Analyst 2015, 140(20), 6782-6798.
Arndt, J. R.; Ghassabi Kondalaji1, S.; Maurer, M. M.; Parker, A.; Legleiter, J.; Valentine, S. J. Huntingtin N-terminal monomeric and multimeric structures destabilized by covalent modification of heteroatomic residues. Biochemistry 2015, 54 (28), 4285-4296.
Donohoe, G. C.; Arndt, J. R.; Valentine, S. J. Online deuterium hydrogen exchange and protein digestion coupled with ion mobility spectrometry and tandem mass spectrometry. Anal. Chem., 2015, 87, 5247-5254.
Khakinjad, M.; Ghassabi-Kondalajii, S.; Tafreshian, A.; Valentine, S. J. Gas-phase hydrogen-deuterium exchange labeling of select peptide ion conformer types: a per residue kinetics analysis. J. Am. Soc. Mass Spectrom, 2015, 26(7), 1115-1127.
Arndt, J. R.; Brown, R. J.; Burke, K.; Legleiter, J.; Valentine, S. J. Lysine residues in the N-terminal huntingtin amphipathic α-helix play a key role in peptide aggregation. J. Mass Spectrom. 2015, 50(1),117-126.
Donohoe G. C.; Maleki, H.; Arndt, J. R.; Khakinejad, M.; Yi, J.; McBride, C.; Nurkiewicz, T. R.; Valentine, S. J. A new ion mobility-linear ion trap instrument for complex mixture analysis. Anal. Chem. 2014, 86(16), 8121-8128.
Khakinejad, M.; Kondalaji, S. G.; Maleki, H.; Arndt, J. R.; Donohoe, G. C.; Valentine, S. J. Combining ion mobility spectrometry with hydrogen-deuterium exchange and top-down MS for peptide ion structure analysis. J Am. Soc. Mass Spectrom. 2014, 25(12), 2103-15.
Donohoe, G. C.; Khakinejad, M.; Valentine, S. J. Ion Mobility Spectrometry-Hydrogen Deuterium Exchange Mass Spectrometry of Anions: Part 1. Peptides to Proteins. J. Am. Soc. Mass Spectrom. 2014, 26(4), 564-576.
Dilger, J. M.; Valentine, S. J.; Glover, M. S; Clemmer, D. E. A Database of Alkaline-earth-coordinated Peptide Cross Sections: Insights into General Aspects of Structure. J. Am. Soc. Mass Spectrom. 2013, 24, 768-779.
Ewing, M. A.; Zucker, S. M.; Valentine, S. J.; Clemmer, D. E. Overtone Mobility Spectrometry: Part 5. Simulations and Analytical Expressions Describing Overtone Limits. J. Am. Soc. Mass Spectrom. 2013, 24, 615-621.
Holliday, A.; Atlasevich, N.; Myung, S.; Plasencia, M. D.; Valentine, S. J.; Clemmer, D. E. Oscillations for Chiral Preference in Proline Clusters. J. Phys. Chem. A., 2013, 117, 1035-1041.
Gaye, M. M.; Valentine, S. J.; Hu, Y.; Mirjankar, N.; Hammoud, Z. T.; Mechref, Y.; Lavine, B. K.; Clemmer, D. E. “Ion Mobility-Mass Spectrometry Analysis of Serum N-Linked Glycans from Esophageal Adenocarcinoma Phenotypes”, J. Prot. Res. 2012, dx.doi.org/10.1021/pr300756e.
Zhu, F.; Lee, S.; Valentine, S. J.; Reilly, J. P.; Clemmer, D. E. “Mannose7 Glycan Isomer Characterization by IMS-MS/MS Analysis”, J. Am. Soc. Mass Spectrom. 2012, DOI: 10.1007/s13361-012-0491-y
Atlasevich, N.; Holliday, A.; Valentine, S. J.; Clemmer, D. E., “Chirality and Packing in Small Proline Clusters”, J. Phys. Chem. B 2012, 116, 11442-11446.
Dilger, J. M.; Valentine, S. J.; Glover, M. S.; Ewing, M. A.; Clemmer, D. E. “A Database of Alkali Metal-Containing Peptide Cross Sections: Influence of Metals on Size Parameters for Specific Amino Acids”, Int. J. Mass Spectrom. 2012, (Special Issue in honor of Peter Armentrout’s 60th birthday), 330-332, 35-45.
Holliday, A.; Atlasevich, N.; Myung, S.; Plasencia, M. D.; Valentine, S. J.; Clemmer, D. E. “Oscillations for Chiral Preference in Proline Clusters”, J. Phys. Chem. A. 2012, dx.doi.org/10.1021/jp302677n.
Atlasevich, N.; Holliday, A.; Valentine, S. J.; Clemmer, D. E. “Collisional Activation of [14Pro+2H]2+ Clusters: Chiral Dependence of Evaporation and Fission Processes”, J. Phys. Chem. B. 2012, 116, 7644-7651.
Lee, S.; Julian, R. R.; Valentine, S. J.; Reilly, J. P., Clemmer, D. E. “Biomolecular condensation via ultraviolet excitation in vacuo”, Int. J. Mass Spectrom. 2012, 316-318, 6-11.
Shi, H.; Pierson, N.; Valentine, S.; Clemmer, D.E. “Conformation Types of Ubiquitin [M+8H]8+ Ions from Water: Methanol Solutions: Evidence for the N and A States in Aqueous Solution”, J. Phys. Chem. B 2012, 116, 3344-3352.
Lee, S.; Valentine, S.J.; Reilly, J.P.; Clemmer, D.E. “Analyzing a Mixture of Disaccharides by IMS-VUVPD-MS”, Int. J. Mass Spectrom. 2012, 309, 161-167.
Lee, S.; Valentine, S.J.; Zucker, S.M.; Webber, N.; Reilly, J.P.; Clemmer, D.E. “Extracted Fragment Ion Mobility Distributions: A New Method for Complex Mixture Analysis”, Int. J. Mass Spectrom. 2012, 309, 154-160.
Lee, S.; Valentine, S.J.; Reilly, J.P.; and Clemmer, D.E. “Controlled Formation of Peptide Bonds in the Gas-Phase”, J. Am. Chem. Soc. 2011, 133(40), 15834-15837.
Zucker, S.M.; Lee, S.; Webber, N.; Valentine, S.J.; Reilly, J.P. and Clemmer, D.E. “An Ion Mobility/Ion Trap/Photodissociation Instrument for Characterization of Ion Structure”, J. Am. Soc. Mass Spectrom. 2011, DOI: 10.1007/s13361-011-0179-8.
Kurulugama, R. T.; Nachtigall, F. M.; Valentine, S. J.; Clemmer, D.E. “Overtone Mobility Spectrometry: Part 4. OMS-OMS Analyses of Complex Mixtures” J. Am. Soc. Mass Spectrom. 2011, 22, 2049-2060.
Pierson, N.A.; Chen, L.; Valentine, S.J.; Russell, D.H.; Clemmer, D.E. “Number of Solution States of Bradykinin from Ion Mobility and Mass Spectrometry Measurements” J. Am. Chem. Soc. 2011, 133 (35), 13810-13813.
Valentine, S.J.; Ewing, M.; Dilger, J.M.; Glover, M.; Geromanos, S.; Hughes, C.; Clemmer, D.E. Using Ion Mobility Data to Improve Peptide Identification: Cross Section Databases and Intrinsic Amino Acid Size Parameters. J. Proteome Res. 2011, 10(5), 2318-2329.
Valentine, S.J.; Kurulugama, R.T.; Clemmer, D. E. “Overtone Mobility Spectrometry: Part 3. On the Origin of Peaks”, J. Am. Soc. Mass Spectrom. 2011, 22(5), 804-16.
Li, Z.; Valentine, S.J.; Clemmer, D.E. “Complexation of Amino Compounds by 18C6 Improves Selectivity by IMS-IMS-MS: Application to Petroleum Characterization”, J Am. Soc. Mass Spectrom. 2011, 22(5), 817-27.
Kim, T.-Y.; Valentine, S. J.; Clemmer, D. E.; Reilly, J. P. “Gas-Phase Conformation-Specific Photofragmentation of Proline-Containing Peptide Ions”, J. Am. Soc. Mass Spectrom. 2010, 21(8), 1455-1465.
Glaskin, R. S.; Valentine, S. J.; Clemmer, D. E. “A Scanning Frequency Mode for Ion Cyclotron Mobility Spectrometry”, Anal. Chem. 2010, 82(19), 8266-8271.
Lee, S.; Ewing, M. A.; Nachtigall, F. M.; Kurulugama, R. T.; Valentine, S. J.; Clemmer, D. E. “Determination of Cross Sections by Overtone Mobility Spectrometry: Evidence for Loss of Unstable Structures at Higher Overtones”, J. Phys. Chem. B 2010, 114(38), 12406-12415.
Pierson, N.; Valentine, S. J.; Clemmer, D. E. “Evidence for a Quasi-Equilibrium Distribution of States for Bradykinin [M+3H]3+ Ions in the Gas Phase” J. Phys. Chem. B 2010, 114 (23), 7777-7783.
Valentine, S. J.; Clemmer, D. E. “Protein oligomers frozen in time” Nature Chemistry 2009, 1 (4), 257-258.